Romania’s National Recovery and Resilience Plan
Componenta 9 PRIVATE SECTOR, RESEARCH, DEVELOPMENT AND INNOVATION SUPPORT
“I8. Development of a program to attract highly specialised human resources from abroad in research, development and innovation activities” PNRR/2022/C9/MCID/I8
CF 190/26.11.2022, contract 760110
Magnetic resonance methods to assess cellular metabolism and the formation of reactive
oxygen species in the context of therapeutic approaches
Project cod: PNRR-III-C9-2022-I8, CF 190/26.11.2023 (contract nr.760110)
Project Program: Romania’s National Recovery and Resilience Plan
Project director: Prof. Arnaud Comment
Project type: Pillar III. Smart, sustainable and inclusive growth, including economic cohesion, jobs, productivity, competitiveness, research, development, and innovation, and a well-functioning internal market with strong small and medium-sized enterprises (SMEs) Component C9. SUPPORT FOR THE PRIVATE SECTOR, RESEARCH, DEVELOPMENT AND INNOVATION
“I8. Development of a program to attract highly specialised human resources from abroad in research, development and innovation activities”
Start date: 01 July 2023
End date: 30 June 2026
Project abstract:
Modern nuclear magnetic resonance methods will be developed for molecule-based experiments in chemistry and biochemistry, with the aim of translation to the clinic. The proposed methods are centered on metabolic biomarkers, especially molecules related to oxidative stress. Their observation by magnetic resonance will be optimized in vitro and in cells to monitor the impact of therapy on metabolism. Magnetic resonance experiments carried out on healthy, tumorous and immune cells within a short time window – on the order of tens of hours - following treatment using drugs or radiation will enable the evaluation of the efficacy and the toxicity of specific therapies. When translated to non-invasive clinical MRI, the proposed biomarkers will be able to yield crucial information on disease evolution in real time, thus allowing treatment optimization.
O1. In vitro observation of enzyme kinetics relevant to metabolites related to oxidative metabolic processes: GSH/GSSG turnover, free-radical production with adapted spin traps and scavenging.
O2. Methods to adapt NMR observation time window to kinetics relevant to the transformation of metabolic substrates such as glucose, glutamine, VitC, GSH, creatine, and choline.
O3. Combined NMR and biological methods for metabolic follow-up in cancer and immune cells.
O4. Assessment of treatment effects on cancer and immune cell metabolism.
1. Halogen bonded supramolecular assemblies constructed from azulene derivatives and perfluorinated di-/triiodobenzenes, A. Dogaru, A. A. Apostol, C. Maxim, M. Raduca, A. S. Novikov, A. Nicolescu, C. Deleanu, S. Nica, M. Andruh, CrystEngComm, 2023,25, 5243-5261.
2. Earth’s field NMR relaxation of pre-polarised water protons for real-time detection of free-radical formation, A. Topor, M. A. Voda, P. Vasos, Chem. Commun., 2023, 59, 11672 - 11675.
3. Improved detection of magnetic interactions in proteins based on long-lived coherences, O. Ianc, F. Teleanu, A. Ciumeică, A. Lupulescu, A. Sadet. P. R. Vasos, Communications Chemistry, 2024, 7, 112, https://www.nature.com/articles/s42004-024-01195-2
4. NMR Spectroscopy in diagnosis and monitoring of methylmalonic and propionic acidemias, C. Deleanu, A. Nicolescu, Biomolecules, 2024,
Proiectul a fost promovat in cadrul vizitei un grup de 14 studenti de la centru colegial de transfer tehnologic Cteau, atașat la Cégep de Saint-Laurent, Canada, condus de Prof. Letitia Gruia.
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